acridine orange fluorescence

by on December 2, 2020

doi: 10.1080/14756366.2017.1292263, 44. Under specific conditions of ionic strength and concentration, acridine orange emits red fluorescence when it binds to RNA by stacking interactions, and green fluorescence when it binds to DNA by intercalation. doi: 10.1002/jso.21025, 24. Kusuzaki K, Takai T, Yoshimura H, Inoue K, Takai S, Baldini N. Clinical trial of radiotherapy after intravenous injection of acridine orange for patients with cancer. In the same study, subcutaneous administration of AO lead to two of thirty mice and one of twenty rats developing tumors at the injection site. Future drug developments include nanoparticles and targeted multipurpose theranostic drugs which can be used for intraoperative guidance and subsequent precision treatment of any microscopic residual tumor (36). Acridine orange is recommended for the u… This study concluded that trained surgeons were able to distinguish between normal and malignant ovarian surface epithelium in AO stained optical biopsies and that accuracy was similar during the ex vivo and in vivo imaging (40). Additionally, the method of acridine orange direct count (AODC) proved useful in the enumeration of bacteria found within landfills. At low pH (inside the organelles), it will emit an orange fluorescence (peak at 590 nm). (2012) 10:164–8. In this paper, we review emergent research about novel applications of AO for fluorescence surgery and cancer therapy. Nakamura T, Kusuzaki K, Matsubara T, Matsumine A, Asanuma K, Satonaka H, et al. doi: 10.3892/or_00000193, 58. The intraoperative direct analysis that CLE can provide, particularly with AO as a contrast agent, has also been studied in the ophthalmological setting. This spectrum was collected by in the summer of 1995 using a Spex FluoroMax. doi: 10.3171/2013.11.FOCUS13486, 39. acridine orange counts. [3], Acridine orange has been widely accepted and used in many different areas, such as epifluorescence microscopy, and the assessment of sperm chromatin quality. In this study AO was used under FDA Investigational New Drug approval at a dose of ≤ 1 μL of 330 μM/L applied topically to the surface of the ovary just prior to imaging (40). Unique spectral signatures of the nucleic acid dye acridine orange can distinguish cell death by apoptosis and necroptosis. doi: 10.1016/j.ajo.2016.06.001, 48. In this study, two patients had sarcomas, two had pancreatic cancer, and one patient each had intrahepatic cholangiocarcinoma, renal, lung, and parotid cancer. With the evidence presented in this review regarding the role of AO in tumor identification, we are especially interested with respect to brain lesions what AO can possibly provide in terms of specific fluorescent tumor labeling. Gareau D, Bar A, Snaveley N, Lee K, Chen N, Swanson N, et al. While some patients benefited in these small pilot studies, further clinical investigation is necessary to assess if the observed cytocidal effect could be achieved in other types of cancer. (2018) 114:e1310–5. AO produces orange fluorescence when it binds to RNA and green fluorescence when it binds to DNA. RDT with AO shows a similar effect on tumor cells when compared to PDT (52). [7] Acridine orange is recommended for the use of fluorescent microscopic detection of microorganisms in smears prepared from clinical and non-clinical materials. Anticancer Res. doi: 10.1111/j.1699-0463.1977.tb01970.x, 6. Int J Gynecol Cancer. Krishnamurthy et al. Belykh E, Martirosyan NL, Yagmurlu K, Miller EJ, Eschbacher JM, Izadyyazdanabadi M, et al. (2005) 25:1225–35. Place a clean lint-free 32-mm coverslip over the mixture. Differential staining of AO has been used to differentiate gliosis and glial neoplasms in human specimens with malignant glial cells demonstrating increased red fluorescence secondary to cytoplasmic RNA accumulation compared to reactive glial cells which remained green (27). Anticancer Res. Outlining Acridine Orange and Propidium Iodide. Clin Orthop Relat Res. (1991) 194:147–53. 22. doi: 10.1016/j.ultrasmedbio.2018.10.016, 61. Total tumor cell elimination with minimum damage to normal tissues in musculoskeletal sarcomas following photodynamic therapy with acridine orange. Acridine orange is used in epifluorescence microscopy and flow cytometry. SUMMARY AND EXPLANATION In 1977, Kronvall and Myhre described the use of Acridine Orange stain to detect microorganisms in direct smears Anticancer Res. Other applications of acridine orange include the simple and sensitive post-mortem detection of early myocardial infarction—normal myocardium fluoresces a golden brown color and myocardial anoxia/ischemia-damaged cells show a shift toward yellow or yellow-green (30). Modulating the Electron‐Donating Ability of Acridine Donor Units for Orange–Red Thermally Activated Delayed Fluorescence Emitters Tianheng Chen Renmin Hospital of Wuhan University, Hubei Key Lab on Organic and Polymeric Optoelectronic Materials, Department of Chemistry, Wuhan University, Wuhan, 430072 P.R. This method is based on the doi: 10.21873/anticanres.12248, 14. (2016) 3:55. doi: 10.3389/fsurg.2016.00055, 38. Copyright © 2019 Byvaltsev, Bardonova, Onaka, Polkin, Ochkal, Shepelev, Aliyev and Potapov. Generally, these images are similar. (2007) 21:205–14. J Cell Biol. However, this behavior differs significantly in live, apoptotic, and fixed permeabilized cells (6). (1969) 23:587–90. Further clinical studies are necessary to more definitively assess the clinical benefit AO-based fluorescence guidance, therapy for sarcomas, and to establish feasibility of this new approach for the treatment of other tumor types. Ex vivo confocal fluorescence microscopy for rapid evaluation of tissues in surgical pathology practice. J Neurosurg. 16. Martirosyan NL, Georges J, Eschbacher JM, Cavalcanti DD, Elhadi AM, Abdelwahab MG, et al. Proc R Soc B. 26. Serum AO levels decreased rapidly within 30 min and were below the detection limit (5 ng/ml) 2 h after intravenous administration (20). Rapid virtual hematoxylin and eosin histology of breast tissue specimens using a compact fluorescence nonlinear microscope. Ethanol exposure during development can have devastating effects on the fetus. After subtotal resection of the tumor, the area of operation was filled with a solution of AO at a concentration of 1 μg/ml and after 5 min, the excess dye was washed off with saline. In dogs, IV administration of AO at 0.1 mg/kg showed no clinical signs of toxicity and no abnormalities were seen in the blood within 30 days (20). Fotia C, Avnet S, Kusuzaki K, Roncuzzi L, Baldini N. Acridine orange is an effective anti-cancer drug that affects mitochondrial function in osteosarcoma cells. doi: 10.1371/journal.pone.0041760, 40. Ann N Y Acad Sci. Injection site of the tail vein also emits green fluorescence Figure adapted with permission from: Kusuzaki et al. However, this behavior differs significantl… Oncol. The use of AO in oncology represents a novel approach but the number of studies is currently limited, likely due to the historical concerns about AO safety. Acridine orange staining has to be performed at an acidic pH to obtain the differential staining, which allows bacterial cells to stain orange and tissue components to stain yellow or green. Neurosurg Focus. In determining the dose/toxicity relationship, Tatsuta et al. However, it is our impression that dying cells are first visible in the red channel. Curr Pharm Des. J Neurooncol. In: IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. Basic Orange 14 Suehiro S, Ohnishi T, Yamashita D, Kohno S, Inoue A, Nishikawa M, et al. *Correspondence: Vadim A. Byvaltsev, byval75vadim@yandex.ru, Front. Mahmoudi K, Garvey KL, Bouras A, Cramer G, Stepp H, Jesu Raj JG, et al. doi: 10.1016/j.clineuro.2018.03.015, 30. The goal of this paper is to review the current literature on the use of AO for fluorescence-guided surgery and cancer therapy. developed a specialized test with sperm AO fluorescence staining to determine male fertility (31). This technology was used to remove breast tumors (35), soft tissue tumors and sarcomas (26, 44, 45), treatment of skin cancer (34, 46), conjunctival tumors (47), renal tumors, lungs, liver (32). doi: 10.1620/tjem.144.265. Delic J, Coppey J, Magdelenat H, Coppey-Moisan M. Impossibility of acridine orange intercalation in nuclear DNA of the living cell. A fluorescent dye such as acridine orange absorbs the energy of incoming light. SA16 Our Acridine Orange Stain is used as a fluorescent staining agent to detect the presence of bacteria in blood cultures and other bodily fluids. Intraoperative Photodynamic Surgery (iPDS) with acridine orange for musculoskeletal sarcomas. The technique had distinct advantages over acridine orange whenenumerating bacteria on surfaces which bind acridine orange, such as polystyrene. Malignant cells also contain increased amounts of RNA and DNA compared to normal cells. 25. This energy cannot be accommodated by the dye forever, and so is released. The next step included AO-PDT, a 10 min exposure of the resection cavity to unfiltered light from a xenon lamp with a brightness of 100,000 lx mounted on an operating microscope. p < 0.05. Ungraded products supplied by Spectrum are indicative of a grade suitable for general industrial use or rese Pricing & More Info BD BBL Acridine Orange Stains doi: 10.3109/00016347209156864, 43. The principles of X-ray radiodynamic therapy (RDT) and sonodynamic therapy (SDT) have gained interest because unlike traditional light-based PDT, it has deeper tissue penetration depth. Matsubara T, Kusuzaki K, Matsumine A, Shintani K, Satonaka H, Uchida A. Acridine orange used for photodynamic therapy accumulates in malignant musculoskeletal tumors depending on pH gradient. Received: 15 April 2019; Accepted: 04 September 2019; Published: 24 September 2019. After intravenous or topical application and soaking of the resection cavity in AO solution, researchers observed increased accumulation of AO in tumor tissue. Jain M, Rajadhyaksha M, Nehal K. Implementation of fluorescence confocal mosaicking microscopy by “early adopter” Mohs surgeons and dermatologists: recent progress. (2018) 169:21–28. doi: 10.1007/s11060-019-03103-4, 63. Matsubara et al. The sharp contrast provides a mechanism for counting the number of organisms present in a sample. The effect of AO fluorescence guidance has not yet been studied in brain tumors and should be tested in comparison with other fluorescence guidance techniques such as 5-aminolevulinic acid, fluorescein sodium, and indocyanine green, which are already available and have a safety record and established efficacy. Translational research of photodynamic therapy with acridine orange which targets cancer acidity. Acta Cytol 1964;8:224-33. Rhoduline Orange N (2014) 6:e204. 51. Udovich JA, Besselsen DG, Gmitro AF. Nat Commun. Clinical outcome of a novel photodynamic therapy technique using acridine orange for synovial sarcomas. Int J Appl Res Vet Med. AO (C17H19N3) is a low molecular weight (265.36 g/mol), weakly basic dye that easily penetrates cell membranes. Tumor destruction due to acridine orange photoactivation by argon laser. Acridine Orange fluorescence spectra Acridine Orange is a cell-permeable dye that has a dominant absorption peak at 460 nm (Fig. (D) Macroscopic fluorescence view of mouse osteosarcoma subcutaneously inoculated in the back of nude mouse after blue light excitation at 2 h after AO injection through the tail vein. Fluorescent light-up acridine orange derivatives bind and stabilize KRAS-22RT G-quadruplex Biochimie. Cancer Res. doi: 10.1093/jac/47.1.1, 5. The data interval was 0.5 nm and the integration time was 2.0 sec. doi: 10.7759/cureus.204. Additionally, acridine orange may be used on blood samples causing bacterial DNA to fluoresce, aiding in the clinical diagnosis of bacterial infections, such as meningitis. AO is permeable to both live and dead cells and stains all nucleated cells to generate green fluorescence. 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acridine orange fluorescence